Difference between revisions of "Part:BBa K1378003"
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− | <figure style="text-align: center"><img style="width: | + | <figure style="text-align: center"><img style="width:30%" src="https://static.igem.org/mediawiki/2014/0/03/Peking2014ZZJ_MVN.png" |
/><figcaption style="text-align:left"><b>Figure 1.</b> Solution structure of MVN. MVN consists of 2 domains, domain A | /><figcaption style="text-align:left"><b>Figure 1.</b> Solution structure of MVN. MVN consists of 2 domains, domain A | ||
and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is | and domain B, which are colored yellow and blue respectively. Domain A is formed by residues 38-93 while domain B is |
Revision as of 16:50, 16 October 2014
Microvirin (MVN)---a lectin from Microcystis aeruginosa
Introduction
This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Microcystis aeruginosa PCC7806 specifically.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 23
Usage and Biology
MVN is a lectin isolated from the cyanobacteria Microcystis aeruginosa PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues [1]. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of M. aeruginosa PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in E. coli and its binding effect to cyanobacteria cells is species-specific [1].