Difference between revisions of "Part:BBa K1381015"

Latest revision as of 16:45, 16 October 2014

Silencing sRNA (15 bases long) for USP45 (Based on Spot42)

This is a mutagenesis of the antisense site of the small RNA Spot42. It contains the J23101 promoter.

Biology

The sRNA has its base from the gene spot42. It consists of an antisense region that recognizes a specific sequence and another region that recruits the protein Hfq. The Hfq protein blocks and prevents the targeted mRNA from binding to the ribosome. This will stop the translation of the USP45 protein complex and no protein should be produced. We have redesigned the antisense region so it will specifically recognize our USP45. The antisense region is 15 nucleotides long. The antisense region will make it bind to our USP45 and recruit the Hfq protein to stick on that region. USP45 is a secretion tag that we coupled to colicins Fy. However, in theory it should work with anything you couple to USP45.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 It contains the [https://parts.igem.org/Part:BBa_J23101 J23101 promoter].
 <h2>Biology</h2>
-The sRNA has its base from the gene spot42. It consists of an antisense region that recognizes a specific sequence and another region that recruits the protein Hfq. The Hfq protein blocks and prevents the targeted mRNA from binding to the ribosome. This will stop the translation of the USP45 protein complex and no protein product should be produced.
+The sRNA has its base from the gene spot42. It consists of an antisense region that recognizes a specific sequence and another region that recruits the protein Hfq. The Hfq protein blocks and prevents the targeted mRNA from binding to the ribosome. This will stop the translation of the USP45 protein complex and no protein should be produced.
-We have redesigned the antisense region so it will specifically recognize our USP45. The antisense region is 15 nucleotides long. The antisense region will make it bind to our USP45 and recruit the Hfq protein to stick on that region. USP45 is a secretion tag that we coupled with [https://parts.igem.org/Part:BBa_K1381018 colicins Fy]. But in theory it should work with whatever you couple to USP45.
+We have redesigned the antisense region so it will specifically recognize our USP45. The antisense region is 15 nucleotides long. The antisense region will make it bind to our USP45 and recruit the Hfq protein to stick on that region. USP45 is a secretion tag that we coupled to [https://parts.igem.org/Part:BBa_K1381018 colicins Fy]. However, in theory it should work with anything you couple to USP45.
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