Difference between revisions of "Part:BBa K1316012"

 
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<partinfo>BBa_K1316012 short</partinfo>
 
<partinfo>BBa_K1316012 short</partinfo>
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Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage
 
Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage
 
MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis
 
MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis
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<h3> Improvement of a part </h3>
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We could not detect the coding sequence of mtrCAB by restriction analysis of Bielefeld 2013 iGEM team part BBa_K1172401 or by sequencing of Bielefeld 2013 iGEM team part BBa_K1172403 (mtrCAB with medium Promoter and medium RBS), which should contain the mtrCAB coding region of BBa_K1172401  according to Bielefeld 2013 iGEM team.
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Our team has created part BBa_K1316012 which contains the proper mtrCAB coding sequence on it and, therefore represents an improvement of BBa_K1172401 and BBa_K1172403 Bielefeld 2013 BioBricks.
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<h3> Characterisation </h3>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:08, 16 October 2014

T7 lacO + mtrCAB

Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis

Improvement of a part

We could not detect the coding sequence of mtrCAB by restriction analysis of Bielefeld 2013 iGEM team part BBa_K1172401 or by sequencing of Bielefeld 2013 iGEM team part BBa_K1172403 (mtrCAB with medium Promoter and medium RBS), which should contain the mtrCAB coding region of BBa_K1172401 according to Bielefeld 2013 iGEM team. Our team has created part BBa_K1316012 which contains the proper mtrCAB coding sequence on it and, therefore represents an improvement of BBa_K1172401 and BBa_K1172403 Bielefeld 2013 BioBricks.

Characterisation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 361