Difference between revisions of "Part:BBa I742130:Experience"

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===Applications of BBa_I742130===
 
===Applications of BBa_I742130===
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===Glasgow 2014 experience===
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We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520].
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We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520]. This can be seen in Fig. 1 below.
  
 
It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560].
 
It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560].
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[[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs downstream of rfp gene transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 K1463000]]]
  
 
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
 
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
  
 
BBa_I742130  translation initiation rate 495.33
 
BBa_I742130  translation initiation rate 495.33
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BBa_K1463560  translation initiation rate 12,204.43
 
BBa_K1463560  translation initiation rate 12,204.43
  

Revision as of 15:40, 16 October 2014


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Applications of BBa_I742130

Glasgow 2014 experience

We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick BBa_K1463520. This can be seen in Fig. 1 below.

It gave a lower fluorescence than our designed reverse RBS BBa_K1463560.

Fig. 1: Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs downstream of rfp gene transcribed from the PhiC31 integrase switch K1463000

Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.

BBa_I742130 translation initiation rate 495.33

BBa_K1463560 translation initiation rate 12,204.43

User Reviews

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