Difference between revisions of "Part:BBa K1463560"
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===Usage and Biology=== | ===Usage and Biology=== | ||
This has been used to drive expression of the reversed RFP part [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] in our phiC31 integrase-based inversion switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000]. | This has been used to drive expression of the reversed RFP part [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] in our phiC31 integrase-based inversion switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000]. | ||
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[[File:Salis-rev-rbs.jpg]] | [[File:Salis-rev-rbs.jpg]] | ||
| + | As can be seen, the designed RBS gives the start codon at position 39 a calculated translation initiation rate of "12204.43". This is 25 fold higher than the calculated translation initiation rate of [https://parts.igem.org/Part:BBa_I742130 BBa_I742130]. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 14:33, 16 October 2014
Reverse RBS
Reverse ribosome binding site designed using the Salis ribosome binding site calculator to have a strength of 12,204 when placed downstream of the reversed rfp part BBa_K1463520
Usage and Biology
This has been used to drive expression of the reversed RFP part BBa_K1463520 in our phiC31 integrase-based inversion switch BBa_K1463000.
It gave a higher level of fluorescence than a previous design for a reverse RBS BBa_I742130
It was designed using the Salis RBS calculator software using the RBS sequence NNNNNNNNNNCTCTAGTA (10 Ns followed by a reversed scar) upstream of the RFP open reading frame.
As can be seen, the designed RBS gives the start codon at position 39 a calculated translation initiation rate of "12204.43". This is 25 fold higher than the calculated translation initiation rate of BBa_I742130. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]