Difference between revisions of "Part:BBa K1317002:Design"

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The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.
 
The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.
  
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[[File:Bdx2014 SLP design01.png|900px]]
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[[File:Bdx2014_SLP_design01_a.png|900px]]
  
 
===Source===
 
===Source===

Revision as of 14:19, 16 October 2014

CDS of silk-like protein (SLP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.

Bdx2014 SLP design01 a.png

Source

major ampullate spidroin 1 (MaSp1) gene from Nephila clavipes

References

[1] https://www.neb.com/tools-and-resources/feature-articles/gibson-assembly-building-a-synthetic-biology-toolset

[2] Shevchuk N.A. et al. Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously (2004) Nucleic Acids Res., 32(2), 19