Difference between revisions of "Part:BBa K1317002:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1317002 short</partinfo> | <partinfo>BBa_K1317002 short</partinfo> | ||
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The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling. | The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling. | ||
− | + | [[File:Bdx2014 SLP design01.png|900px]] | |
===Source=== | ===Source=== | ||
− | major ampullate spidroin 1 (MaSp1) gene from Nephila clavipes | + | major ampullate spidroin 1 (MaSp1) gene from ''Nephila clavipes'' |
===References=== | ===References=== | ||
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+ | [1] https://www.neb.com/tools-and-resources/feature-articles/gibson-assembly-building-a-synthetic-biology-toolset | ||
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+ | [2] Shevchuk N.A. et al. ''Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously'' (2004) Nucleic Acids Res., 32(2), 19 |
Revision as of 14:16, 16 October 2014
CDS of silk-like protein (SLP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 312
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.
Source
major ampullate spidroin 1 (MaSp1) gene from Nephila clavipes
References
[2] Shevchuk N.A. et al. Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously (2004) Nucleic Acids Res., 32(2), 19