Difference between revisions of "Part:BBa K1342001"
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fig.2
 
fig.2
 
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intensity of the band which is the Quantitative data of the aspartic acid synthesis.]]
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Estimation of aspartic acid content in spot
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Each intensity of spots indicating the content of aspartic acid, was estimated by using Image J.
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.]]

Revision as of 12:58, 16 October 2014

zinTp_AspA


Asp Device image.jpg


ZinTp_AspA contains ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002) and Dter(BBa_B0015). The AspA encoding aspartase that synthesize aspartic acid from fumaric acid and ammonium, is expressed in the presence of cadmium ion.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 600
    Illegal AgeI site found at 1452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1111



TLC Assay

Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)


fig.2
Estimation of aspartic acid content in spot Each intensity of spots indicating the content of aspartic acid, was estimated by using Image J. .