Difference between revisions of "Part:BBa K1453000:Design"

 
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<partinfo>BBa_K1453000 short</partinfo>
 
<partinfo>BBa_K1453000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.  
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With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning)  
+
When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.  
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More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai.
+
 
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More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg.
+
With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning)  
 +
 
 +
More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.
 +
 
 +
More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.
  
  

Revision as of 12:53, 16 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158


Design Notes

When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.

With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning)

More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.

More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.


Source

BBa_K771000 BBa_E1010

References