Difference between revisions of "Part:BBa K1453000"
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<partinfo>BBa_K1453000 short</partinfo> | <partinfo>BBa_K1453000 short</partinfo> | ||
− | This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane. | + | This part is designed as a scaffold of the first kind of '''connectee''', which can anchor on the membrane. |
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The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 | The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 | ||
In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI. | In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI. | ||
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Revision as of 12:53, 16 October 2014
ssDsbA-mRFP-Lgt-TAL USB-His Tag
This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.
Structure
The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1158