Difference between revisions of "Part:BBa K1475007"
 
Line 11: Line 11:
 
This part can be used to test the expression from the lacI, p(lacI) system.
 
This part can be used to test the expression from the lacI, p(lacI) system.
 
<br>
 
<br>
−
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by “Escherichia coli” and therefore remains at a constant concentration.
+
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by ''Escherichia coli'' and therefore remains at a constant concentration.
  
  
Line 28: Line 28:
 
1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html  
 
1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html  
 
<br>
 
<br>
−
UniProt accesstion for “Escherichia coli” lacI: http://www.uniprot.org/uniprot/P03023
+
UniProt accesstion for ''Escherichia coli'' lacI: http://www.uniprot.org/uniprot/P03023
 
<br>
 
<br>
−
Entrez gene accession for “Escherichia coli” lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
+
Entrez gene accession for ''Escherichia coli'' lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
 
<br>
 
<br>
−
Protein database entry for “Escherichia coli” lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG
+
Protein database entry for ''Escherichia coli'' lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

Latest revision as of 12:49, 16 October 2014

GFP controlled by lacI and plac

This composite part has GFP controlled by a lacI, p(lacI) expression system.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.


Usage and Biology

This part can be used to test the expression from the lacI, p(lacI) system.
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by Escherichia coli and therefore remains at a constant concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2057



References

1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG