Difference between revisions of "Part:BBa K1475008"
 
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===Usage and Biology===
 
===Usage and Biology===
−
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by “Escherichia coli” and therefore remains at a constant concentration.
+
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by ''Escherichia coli'' and therefore remains at a constant concentration.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 12:48, 16 October 2014

lacI protein produced by constitutive promoter

This part is the lacI repressor controlled by a constitutively active promoter.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.


Usage and Biology

The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by Escherichia coli and therefore remains at a constant concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG