Difference between revisions of "Part:BBa K1475008"

Revision as of 12:47, 16 October 2014

lacI protein produced by constitutive promoter

This part is the lacI repressor controlled by a constitutively active promoter.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.

Usage and Biology

The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by “Escherichia coli” and therefore remains at a constant concentration.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

@@ Line 21: / Line 21: @@
 ===References===
-UniProt accesstion for “Escherichia coli” lacI: http://www.uniprot.org/uniprot/P03023
+UniProt accesstion for ''Escherichia coli'' lacI: http://www.uniprot.org/uniprot/P03023
 <br>
-Entrez gene accession for “Escherichia coli” lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
+Entrez gene accession for ''Escherichia coli'' lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
 <br>
-Protein database entry for “Escherichia coli” lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG
+Protein database entry for ''Escherichia coli'' lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG