Difference between revisions of "Part:BBa K1475009"
 
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<partinfo>BBa_K1475009 short</partinfo>
 
<partinfo>BBa_K1475009 short</partinfo>
  
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This part contains the LIMS1 gene from “Citrus limon” encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with [https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040], or without [https://parts.igem.org/Part:K1475003 Part:K1475003] the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag].
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This part contains the LIMS1 gene from ''Citrus limon'' encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with [https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040], or without [https://parts.igem.org/Part:K1475003 Part:K1475003] the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag].
  
  

Latest revision as of 12:46, 16 October 2014

Limonene synthase with tet promoter, RBS and terminator

This part contains the LIMS1 gene from Citrus limon encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with Part:BBa_C0040, or without Part:K1475003 the LVA rapid degradation tag.


Usage and Biology

The LIMS1 gene encodes the (R)-limonene synthase from Citrus limon. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [2]
(R)-limonene is the main component of lemon scent and is for instance used in food manufacturing.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178



References

1. UniProt, accession number: Q8L5K3. http://www.uniprot.org/uniprot/Q8L5K3
2. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0