Difference between revisions of "Part:BBa K1475009:Design"
Line 16: Line 16:
 
===Source===
 
===Source===
  
−
Organizm: ”Citrus limon”
+
Organizm: "Citrus limon"
  
 
===References===
 
===References===

Revision as of 12:44, 16 October 2014

Limonene synthase with tet promoter, RBS and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


Design Notes

The device was constructed using standard assembly from the p(TetR) repressible promoter Part:BBa_R0040 and a LIMS1 translational unit Part:BBa_I742111

LIMS1: Due to the natural sequence containing several forbidden restriction sites some codons was mutated silently to make the sequence compliant with the BioBrick standard during the initial design of the LIMS1 basic part (Part:BBa_I742110).



Source

Organizm: "Citrus limon"

References

1. UniProt, accession number: Q8L5K3. http://www.uniprot.org/uniprot/Q8L5K3
2. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0