Difference between revisions of "Part:BBa K1475009"

 
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__NOTOC__
 
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<partinfo>BBa_K1475009 short</partinfo>
 
<partinfo>BBa_K1475009 short</partinfo>
  
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Limonene synthase from Citrus limon regulated by the tet promoter.  
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This part contains the LIMS1 gene from “Citrus limon” encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with [https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040], or without [https://parts.igem.org/Part:K1475003 Part:K1475003] the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag].
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===Usage and Biology===
 
===Usage and Biology===
  
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The LIMS1 gene encodes the (R)-limonene synthase from “Citrus limon”. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [2]
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<br>
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(R)-limonene is the main component of lemon scent and is for instance used in food manufacturing.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475009 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1475009 parameters</partinfo>
 
<partinfo>BBa_K1475009 parameters</partinfo>
 
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===References===
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1. UniProt, accession number: Q8L5K3. http://www.uniprot.org/uniprot/Q8L5K3
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2. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0

Revision as of 12:42, 16 October 2014

Limonene synthase with tet promoter, RBS and terminator

This part contains the LIMS1 gene from “Citrus limon” encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with Part:BBa_C0040, or without Part:K1475003 the LVA rapid degradation tag.


Usage and Biology

The LIMS1 gene encodes the (R)-limonene synthase from “Citrus limon”. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [2]
(R)-limonene is the main component of lemon scent and is for instance used in food manufacturing.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178



References

1. UniProt, accession number: Q8L5K3. http://www.uniprot.org/uniprot/Q8L5K3 2. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0