Difference between revisions of "Part:BBa K1433008"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1433008 short</partinfo>
 
<partinfo>BBa_K1433008 short</partinfo>
  
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Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination between attB and attP, the attachment sites on the phage chromosome and host chromosome. This recombination result in reverse of the sequence between attB and attP, then form new sites attL and attR. This revert can restore by appropriately controlling the conditional heterologous expression of integrase and an excisionase in Bxb1 named gp47.
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<p>Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination between attB and attP, the attachment sites on phage chromosome and host chromosome. This recombination results in the reverse of the sequence between attB and attP, changing the two sites to attL and attR at the meantime. This inversion can be reversible by appropriately controlling the conditional expression of integrase and an excisionase in Bxb1 named gp47 at certain ratio. We change start codon ATG to GTG for a appropriate expression quantity. </p>
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This part is composed of 3 elements.  
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1. RBS: 6N, a suitable RBS in paper.&#8232;
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[[File:int-xis-bplr.gif]]
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2. gp35: a serine integrase in Mycobacterium phage Bxb1.
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3. terminator: BBa_B0015, a strong double terminator.
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<p><b><big>Composition</big></b><br/>
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<ol><li> RBS: 6N, a suitable RBS in paper (Bonnet, et al. 2012).</li>
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<li>gp35: a serine integrase in Mycobacterium phage Bxb1.</li>
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<li>terminator: BBa_B0015, a strong double terminator.</li>
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</ol></p>
  
  

Revision as of 12:39, 16 October 2014

6N-gp35-Terminator

Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination between attB and attP, the attachment sites on phage chromosome and host chromosome. This recombination results in the reverse of the sequence between attB and attP, changing the two sites to attL and attR at the meantime. This inversion can be reversible by appropriately controlling the conditional expression of integrase and an excisionase in Bxb1 named gp47 at certain ratio. We change start codon ATG to GTG for a appropriate expression quantity.

File:Int-xis-bplr.gif

Composition

  1. RBS: 6N, a suitable RBS in paper (Bonnet, et al. 2012).
  2. gp35: a serine integrase in Mycobacterium phage Bxb1.
  3. terminator: BBa_B0015, a strong double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
    Illegal NheI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 491
    Illegal XhoI site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1130
    Illegal NgoMIV site found at 1217
    Illegal AgeI site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325