Difference between revisions of "Part:BBa K1475007"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1475007 short</partinfo>
 
<partinfo>BBa_K1475007 short</partinfo>
  
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This device has GFP controlled by the lacI repressor and lac promoter system.
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This composite part has GFP controlled by a lacI, p(lacI) expression system.
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<br>
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Please note that this part contains the lacI repressor without the LVA rapid [https://parts.igem.org/Protein_domains/Degradation degradation tag]. The lacI+LVA bacis part can be found here: [https://parts.igem.org/Part:BBa_C0012 Part:BBa_C0012].
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part can be used to test the expression from the lacI, p(lacI) system.
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<br>
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The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by “Escherichia coli” and therefore remains at a constant concentration.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1475007 parameters</partinfo>
 
<partinfo>BBa_K1475007 parameters</partinfo>
 
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<!-- -->
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===References===
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1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
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<br>
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UniProt accesstion for “Escherichia coli” lacI: http://www.uniprot.org/uniprot/P03023
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<br>
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Entrez gene accession for “Escherichia coli” lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
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<br>
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Protein database entry for “Escherichia coli” lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

Revision as of 12:36, 16 October 2014

GFP controlled by lacI and plac

This composite part has GFP controlled by a lacI, p(lacI) expression system.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.


Usage and Biology

This part can be used to test the expression from the lacI, p(lacI) system.
The operation of lacI repressor is inhibited by allolactose and [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)]. IPTG is preferred because it cannot be degraded by “Escherichia coli” and therefore remains at a constant concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2057



References

1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
UniProt accesstion for “Escherichia coli” lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for “Escherichia coli” lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for “Escherichia coli” lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG