Difference between revisions of "Part:BBa K1475004"

 
 
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<partinfo>BBa_K1475004 short</partinfo>
 
<partinfo>BBa_K1475004 short</partinfo>
  
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This device will produce the TetR protein (Part:BBa_K1475003) using a constitutive promoter (Part:BBa_J23106) and RBS (Part:BBa_B0030) followed by a small artificial terminator (Part:BBa_B1002).
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The tetracycline repressor (TetR) expressed using a constitutive promoter.
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TetR represses transcription on binding to the p(TetR) promoter [https://parts.igem.org/Part:BBa_R0040 Part:BBa_R0040]. [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] binds to the Tet repressor inhibiting its function and hereby releasing the repression on the Tet promoter. [1]
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===Usage and Biology===
 
===Usage and Biology===
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In addition to ATc (anhydrotetracycline), dox (Doxycycline) can also be used as inducer. [2]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475004 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1475004 parameters</partinfo>
 
<partinfo>BBa_K1475004 parameters</partinfo>
 
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===References===
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1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
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<br>
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2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components

Latest revision as of 12:19, 16 October 2014

Cons. promoter, RBS, TetR and a double terminator

The tetracycline repressor (TetR) expressed using a constitutive promoter. TetR represses transcription on binding to the p(TetR) promoter Part:BBa_R0040. [http://openwetware.org/wiki/ATc ATc (anhydrotetracycline)] binds to the Tet repressor inhibiting its function and hereby releasing the repression on the Tet promoter. [1]


Usage and Biology

In addition to ATc (anhydrotetracycline), dox (Doxycycline) can also be used as inducer. [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components