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Gluconacetobacter xylinus ATCC 53582

Gluconacetobacter xylinus is an obligate aerobe, Gram negative bacterial species which produces bacterial cellulose. Strain ATCC 53582 has been reported as one of the highest cellulose-producing strains, and readily produces cellulose with different carbon feedstocks.

Culturing G. xylinus ATCC 53582

G.xylinus ATCC 53582 growth rate varies depending on the culturing conditions. Growth rate is highest when G.xylinus is cultured at 30degC, 180rpm shaking in a well-aerated setting. However, several authors have noted that in shaking conditions, the prevalence of cellulose non-producing mutants (cel- mutants) increases, which causes a decrease in the total yield of produced cellulose. For this reason, when high cellulose production is required, G.xylinus is often grown in static conditions, at 30degC, in a well-aerated setting. Although several different media have been used for culturing G.xylinus, the most commonly used is Hestrin-Schramm (HS) medium. HS medium contains the following components (for a protocol for G.xylinus culturing, see ([G.xylinus media and culturing]): 2% (w/v) Glucose 0.5% (w/v) Yeast extract 0.5% (w/v) Peptone 0.27% (w/v) Na2HPO4 0.15% (w/v) Citric acid

Cellulose productivity of G.xylinus ATCC 53582

Feedstocks for G.xylinus ATCC 53582

Natural antibiotics resistance of G.xylinus ATCC 53582

Sequence and Features