Difference between revisions of "Part:BBa K1342001"
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ZinTp_AspA contains ZinTp([https://parts.igem.org/Part:BBa_K1342005 BBa_K1342005]), RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), ApeA([https://parts.igem.org/Part:BBa_K1342002 BBa_K1342002]), Dter([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
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ZinTp_AspA contains ZinTp([https://parts.igem.org/Part:BBa_K1342005 BBa_K1342005]), RBS([https://parts.igem.org/Part:BBa_B0034 BBa_B0034]), ApeA([https://parts.igem.org/Part:BBa_K1342002 BBa_K1342002]) and Dter([https://parts.igem.org/Part:BBa_B0015 BBa_B0015]).
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AspA is expressed in the presence of cadmium ion. And translated to aspartase.
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The AspA encoding aspartase that synthesize aspartic acid from fumaric acid and ammonium, is expressed in the presence of cadmium ion.  
  
  

Revision as of 12:07, 16 October 2014

zinTp_AspA


Asp Device image.jpg


ZinTp_AspA contains ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002) and Dter(BBa_B0015). The AspA encoding aspartase that synthesize aspartic acid from fumaric acid and ammonium, is expressed in the presence of cadmium ion.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1071
    Illegal AgeI site found at 600
    Illegal AgeI site found at 1452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1111



TLC Assay

Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)


fig.2
intensity of the band which is the Quantitative data of the aspartic acid synthesis.