Difference between revisions of "Part:BBa K1541017"

(Quorum Sensing)
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RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'' which produces the  
 
RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'' which produces the  
  
 +
====RBS Engineering====
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:50, 16 October 2014

rhlI with optimized RBS

N-butyryl-L-Homoserine lactone or short C4-HSL

This part contains the part Part:BBa_C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR, (Part:BBa_C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.

Usage and Biology

Quorum Sensing

RhlI is the autoinducer synthetase from Pseudomonas aeruginosa which produces the

RBS Engineering

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]