Difference between revisions of "Part:BBa K1541017"
| Line 2: | Line 2: | ||
<partinfo>BBa_K1541017 short</partinfo> | <partinfo>BBa_K1541017 short</partinfo> | ||
[[File:ETH_Zurich_2014_C4-HSL.png|thumb| ''N''-butyryl-L-Homoserine lactone or short C4-HSL ]] | [[File:ETH_Zurich_2014_C4-HSL.png|thumb| ''N''-butyryl-L-Homoserine lactone or short C4-HSL ]] | ||
| − | This part contains the part [[Part:BBa_C0170]] (RhlI), which is an autoinducer synthesis protein that produces [[AHL|''N''-butyryl-L-HSL]] (C4-HSL) which binds to RhlR, ([[Part:BBa_C0171]]), obtained from ''Pseudomonas aeruginosa''. | + | This part contains the part [[Part:BBa_C0170]] (RhlI), which is an autoinducer synthesis protein that produces [[AHL|''N''-butyryl-L-HSL]] (C4-HSL) which binds to RhlR, ([[Part:BBa_C0171]]), obtained from ''Pseudomonas aeruginosa''. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of [[AHL|C4-HSL]]. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:44, 16 October 2014
rhlI with optimized RBS
This part contains the part Part:BBa_C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR, (Part:BBa_C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.
Usage and Biology
Quorum Sensing
RhlI is the autoinducer synthetase from Pseudomonas aeruginosa which produces the
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]