Difference between revisions of "Part:BBa K1433003"
Line 6: Line 6:
  
 
<p><b><big>Composition</big></b><br />
 
<p><b><big>Composition</big></b><br />
−
<ol><li> Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.</li>
+
<ol><li> Promoter: [https://parts.igem.org/Part:BBa_J23110 BBa_J23110], a middle-ground Bacterial constitutive promoter.</li>
 
<li>gp47: An excisionase in Mycobacterium phage Bxb1.</li>
 
<li>gp47: An excisionase in Mycobacterium phage Bxb1.</li>
 
<li>tag: A distinct proteolysis tag on gp47 peptides.</li>
 
<li>tag: A distinct proteolysis tag on gp47 peptides.</li>
−
<li>terminator: BBa_B0015, a strong double terminator.</li></ol></p>
+
<li>terminator: [https://parts.igem.org/Part:BBa_B0015 BBa_B0015], a strong double terminator.</li></ol></p>
  
 
[[File: ZJU_K1433003.jpg]]
 
[[File: ZJU_K1433003.jpg]]

Revision as of 11:35, 16 October 2014

RBS-gp47-tag-terminator

Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.

ZJU int xis bplr.gif

Composition

  1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. gp47: An excisionase in Mycobacterium phage Bxb1.
  3. tag: A distinct proteolysis tag on gp47 peptides.
  4. terminator: BBa_B0015, a strong double terminator.

File:ZJU K1433003.jpg

This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 29
    Illegal AgeI site found at 797
  • 1000
    COMPATIBLE WITH RFC[1000]