Difference between revisions of "Part:BBa K1433001"

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<partinfo>BBa_K1433001 short</partinfo>
 
<partinfo>BBa_K1433001 short</partinfo>
  
Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.  
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<p>Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. </p>
  
This part is composed of 2 elements.:
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<p><b><big>composition:</big></b><br />
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<ol>This part is composed of 2 elements.:
 +
<li>Promoter:  [https://parts.igem.org/Part:BBa_J23110 BBa_J23110], a middle-ground Bacterial constitutive promoter.</li>
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<li>attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.</li>
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</ol></p>
  
1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
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[[File:int-xis-bplr-reverse.gif]]
  
2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
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<p>This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.</p>
  
This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:16, 16 October 2014

attB-J23110-attP

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.

composition:

    This part is composed of 2 elements.:
  1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.

File:Int-xis-bplr-reverse.gif

This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23
    Illegal BsaI.rc site found at 106