Difference between revisions of "Part:BBa K1371005"

 
 
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<partinfo>BBa_K1371005 short</partinfo>
 
<partinfo>BBa_K1371005 short</partinfo>
  
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One of parts of the DEBS1
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== '''KR Part'''  ==
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The function of KR domain is deoxidizing the extended unit and enabling it to form the β-hydroxyl ester bond. In KR domain catalyzed reactions, the carbonyl groups of polyketide chains anchored on the ACP domains are combined with the specific binding sites of KR domains through weak chemical bonds. Then the electron cloud in the carbonyl groups is moving towards oxygen atoms so that the carbon atoms have net positive charge. At this time, NADPHs have a chance to bind to the polyketide chains and form instable ternary complexes. In order to reduce the inner energy of this ternary complexes and run towards stable, NADPHs provide hydride ions and KR domains provide protons to the complexes and convert the carbonyl groups to theβ-hydroxyl esters. Finally, the KR domain residues which have lost protons are deoxidized to the initial KR domains by other NADPHs.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:40, 16 October 2014

L3-KR1


KR Part

The function of KR domain is deoxidizing the extended unit and enabling it to form the β-hydroxyl ester bond. In KR domain catalyzed reactions, the carbonyl groups of polyketide chains anchored on the ACP domains are combined with the specific binding sites of KR domains through weak chemical bonds. Then the electron cloud in the carbonyl groups is moving towards oxygen atoms so that the carbon atoms have net positive charge. At this time, NADPHs have a chance to bind to the polyketide chains and form instable ternary complexes. In order to reduce the inner energy of this ternary complexes and run towards stable, NADPHs provide hydride ions and KR domains provide protons to the complexes and convert the carbonyl groups to theβ-hydroxyl esters. Finally, the KR domain residues which have lost protons are deoxidized to the initial KR domains by other NADPHs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal AgeI site found at 159
    Illegal AgeI site found at 229
    Illegal AgeI site found at 856
    Illegal AgeI site found at 1138
  • 1000
    COMPATIBLE WITH RFC[1000]