Difference between revisions of "Part:BBa M0051:Experience"

Revision as of 09:10, 16 October 2014

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Applications of BBa_M0051

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UNIQ924fee7166a792a1-partinfo-00000000-QINU UNIQ924fee7166a792a1-partinfo-00000001-QINU

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iGEM14_Edinburgh

iGEM14_Edinburgh added this tag to GFP (Part:BBa_K1399007)and constructed lactose/IPTG inducible measurement pathway (Part:BBa_K1399017) to characterize and compare the instability of this SsrA-AAV tagged GFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)

SsrA degron tagged GFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).

Summary of BioBricks to which our characterization data is applicable.

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 <I>iGEM14_Edinburgh</I>
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-iGEM14_Edinburgh added this tag to GFP ([[Part:BBa_K1399007]])and constructed lactose/IPTG inducible measurement pathway  ([[Part:BBa_K1399017]]) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)
+iGEM14_Edinburgh added this tag to GFP ([[Part:BBa_K1399007]])and constructed lactose/IPTG inducible measurement pathway  ([[Part:BBa_K1399017]]) to characterize and compare the instability of this SsrA-AAV tagged GFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)
 [[File:EdiGEM14 GFP min and max values at different temperatures.jpg|600px|thumb|center|'''SsrA degron tagged GFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).''']] [[File:EDiGEM14 GFP legend.png|500px|thumb|center|'''Summary of BioBricks to which our characterization data is applicable.''']]
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