Difference between revisions of "Part:BBa M0050:Experience"
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<I>iGEM14_Edinburgh</I> | <I>iGEM14_Edinburgh</I> | ||
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| − | iGEM14_Edinburgh added this tag to RFP ([[Part:BBa_K1399002]]) and GFP ([[Part:BBa_K1399006]])constructed lactose/IPTG inducible measurement pathway ([[Part:BBa_K1399012]] and [[BBa_K1399016]], respectively) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].) | + | iGEM14_Edinburgh added this tag to RFP ([[Part:BBa_K1399002]]) and GFP ([[Part:BBa_K1399006]])constructed lactose/IPTG inducible measurement pathway ([[Part:BBa_K1399012]] and [[Part:BBa_K1399016]], respectively) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].) |
| − | [[File:Tagged RFP min and max values at different temperatures.jpg|600px|thumb|center|'''SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).''']] [[File:EDiGEM14 RFP legend.png|500px|thumb|center|'''Summary of BioBricks to which our characterization data is applicable.''']] | + | [[File:Tagged RFP min and max values at different temperatures.jpg|600px|thumb|center|'''SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).''']] [[File:EDiGEM14 RFP legend.png|500px|thumb|center|'''Summary of BioBricks to which our characterization data is applicable.''']] |
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Revision as of 08:50, 16 October 2014
This experience page is provided so that any user may enter their experience using this part.
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Applications of BBa_M0050
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UNIQ49cb32a019db7f8c-partinfo-00000000-QINU UNIQ49cb32a019db7f8c-partinfo-00000001-QINU
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iGEM14_Edinburgh |
iGEM14_Edinburgh added this tag to RFP (Part:BBa_K1399002) and GFP (Part:BBa_K1399006)constructed lactose/IPTG inducible measurement pathway (Part:BBa_K1399012 and Part:BBa_K1399016, respectively) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].) |
