Difference between revisions of "Part:BBa K1463002"
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<partinfo>BBa_K1463002 short</partinfo>
 
<partinfo>BBa_K1463002 short</partinfo>
  
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PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter. PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in [https://parts.igem.org/Part:BBa_K146300 BBa_K1463001].
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PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter. PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in [https://parts.igem.org/Part:BBa_K1463001 BBa_K1463001].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:30, 16 October 2014

Recombinase Switch With GFP (weaker RBS)

PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter. PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in BBa_K1463001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 105
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 62
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 938