Difference between revisions of "Part:BBa K1373001:Design"
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===Design Notes=== | ===Design Notes=== | ||
cloning via omega-PCR | cloning via omega-PCR | ||
| + | |||
| + | Primers | ||
| + | F-nadE:ctagctactagagaaagaggagaaaATGACATTGCAACAACAAATAATAAAG | ||
| + | R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc | ||
===Source=== | ===Source=== | ||
Revision as of 05:48, 16 October 2014
nadE with strong promter and strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
cloning via omega-PCR
Primers F-nadE:ctagctactagagaaagaggagaaaATGACATTGCAACAACAAATAATAAAG R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc
Source
the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655