Difference between revisions of "Part:BBa K1373000"

Revision as of 05:36, 16 October 2014

This part encodes NAD synthetase

Overexpression of nadE

Electricity active cells (EAC) generate electrons by organic substrate metabolism and transfer them through extracellular transport to an electrode surface in Microbial fuel cell (MFCs). Therefore, genetic modifications that increase the amount of electrons in EAC is one of feasibilities to optimize the electricity power output of MFCs.

Recent studies have proved that the intracellular redox state of EAC is one of the most important physiological traits of extracellular electron transfer efficiency. Particularly, the NAD+(H) pool size plays a central role of most metabolic pathways. In this study, we aim that, overexpression of gene nadE which encodes a NAD synthetase and catalyzes the final step in de novo synthesis and salvage pathway of NAD biosynthesis (Fig. 1), may increase the NAD+ level. Therefore the augmented pool size of NAD+(H) result in promotion of NADH (the carrier of electrons) level, leading to high generation of intracellular releasable electrons and better electricity performance of EAC.

Sequence and Features