Difference between revisions of "Part:BBa K1373001:Design"
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===Design Notes=== | ===Design Notes=== | ||
cloning via omega-PCR | cloning via omega-PCR | ||
| − | + | overexpression intracellular redox state of EAC is one of the most important physiological traits of extracellular electron transfer efficiency.In particular, the NAD+(H) pool size plays a central role of most metabolic pathways. By overexpressing the NAD synthetase, encoded be gene nadE and catalyzes the final step in de novo synthesis and salvage pathway of NAD biosynthesis (Fig. 1), the NAD+ level is increased thereby up-regulating genes whose products catalyze NADH synthesis. Therefore the augmented pool size of NAD+(H) result in promotion of NADH(the carrier of electrons)level, leading to high generation of intracellular releasable electrons and better electricity performance of EAC | |
Revision as of 03:08, 16 October 2014
nadE with strong promter and strong RBS
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
cloning via omega-PCR overexpression intracellular redox state of EAC is one of the most important physiological traits of extracellular electron transfer efficiency.In particular, the NAD+(H) pool size plays a central role of most metabolic pathways. By overexpressing the NAD synthetase, encoded be gene nadE and catalyzes the final step in de novo synthesis and salvage pathway of NAD biosynthesis (Fig. 1), the NAD+ level is increased thereby up-regulating genes whose products catalyze NADH synthesis. Therefore the augmented pool size of NAD+(H) result in promotion of NADH(the carrier of electrons)level, leading to high generation of intracellular releasable electrons and better electricity performance of EAC
Source
the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655