Difference between revisions of "Part:BBa K1462230:Design"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1462230 short</partinfo> | <partinfo>BBa_K1462230 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph. | |
===Source=== | ===Source=== | ||
+ | Clostridium beijerinckii | ||
− | |||
===References=== | ===References=== | ||
+ | Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36. |
Revision as of 02:56, 16 October 2014
Gal1+Crt+cyc1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.
Source
Clostridium beijerinckii
References
Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.