Difference between revisions of "Part:BBa K1462220:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1462220 short</partinfo> | <partinfo>BBa_K1462220 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | + | The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.Hbd,originated from Clostridium beijerinckii, expresses 3-hydroxybutyryl-CoA dehydrogenase, which converts AcAcCoA to HbCoA, the second step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Erg10(BBa_K1462210),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph. | |
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===Source=== | ===Source=== | ||
− | + | Clostridium beijerinckii | |
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===References=== | ===References=== | ||
+ | Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36. |
Revision as of 02:52, 16 October 2014
Gal1+Hbd+ADH1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 942
Design Notes
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.Hbd,originated from Clostridium beijerinckii, expresses 3-hydroxybutyryl-CoA dehydrogenase, which converts AcAcCoA to HbCoA, the second step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Erg10(BBa_K1462210),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph.
Source
Clostridium beijerinckii
References
Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.