Difference between revisions of "Part:BBa K1462210:Design"

 
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<partinfo>BBa_K1462210 short</partinfo>
 
<partinfo>BBa_K1462210 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Erg10,native in Saccharomyces cerevisiae, expresses thiolase, which converts AcCoA to AcAcCoA, the first step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph.
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===Source===
 
===Source===
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===References===
 
===References===
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Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.

Revision as of 02:49, 16 October 2014

Gal1+Erg10+cyc1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Erg10,native in Saccharomyces cerevisiae, expresses thiolase, which converts AcCoA to AcAcCoA, the first step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Crt(BBa_K1462230) in plasmid 181 ,which is leucine auxotroph.

Source

S. cerevisiae genomic sequence

References

Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.