Difference between revisions of "Part:BBa K1462100:Design"

 
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<partinfo>BBa_K1462100 short</partinfo>
 
<partinfo>BBa_K1462100 short</partinfo>
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===Design Notes===
 
===Design Notes===
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TOM22 is fused at the C-terminus of the GFP, and this biobrick is driven by GAL promoter .TOM22 is TOM22 is obtained from saccharomyces cerevisiae by PCR technique.When we constructed this part, we accidentally find that there is a PST1 cleavages lies in the sequence, which cause a problem of ligation. So, we have to knock down the PST1 cleavage. Now, the part is still unded construction
  
 
===Source===
 
===Source===
  
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TOM22 is obtained from saccharomyces cerevisiae by PCR technique, the other elements is from the kit provided by official
  
 
===References===
 
===References===

Revision as of 01:56, 16 October 2014

Gal1+GFP+TOM22+ADH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1468
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1468
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199


Design Notes

TOM22 is fused at the C-terminus of the GFP, and this biobrick is driven by GAL promoter .TOM22 is TOM22 is obtained from saccharomyces cerevisiae by PCR technique.When we constructed this part, we accidentally find that there is a PST1 cleavages lies in the sequence, which cause a problem of ligation. So, we have to knock down the PST1 cleavage. Now, the part is still unded construction

Source

TOM22 is obtained from saccharomyces cerevisiae by PCR technique, the other elements is from the kit provided by official

References