Difference between revisions of "Part:BBa K1462090:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1462090 short</partinfo>
 
<partinfo>BBa_K1462090 short</partinfo>
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===Design Notes===
 
===Design Notes===
 
11
 
11
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　To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP; while the control group was without CoxVI. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.
  
  
 
===Source===
 
===Source===
  
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11
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COXVI is obtained from genome of saccharomyces cerevisiae, the other parts is from the kit provided by official
  
 
===References===
 
===References===

Revision as of 01:43, 16 October 2014

Gal1+cox6+GFP+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325


Design Notes

11 　To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP; while the control group was without CoxVI. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.


Source

COXVI is obtained from genome of saccharomyces cerevisiae, the other parts is from the kit provided by official

References