Difference between revisions of "Part:BBa K1442111"
 
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== DPP-IV ==
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== DPP-IV 3'UTR (target of the siRNA)==
  
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As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).  
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<p>As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113). </p>
  
  
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== Usage ==
 
== Usage ==
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<p>This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.</p>
  
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This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.
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== Model ==
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<p><html><body><img src="https://static.igem.org/mediawiki/parts/e/ec/DR_dppiv_model.PNG"/></body></html></p>
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<p><html><body><img src="https://static.igem.org/mediawiki/parts/2/23/Rds_dppiv_model.PNG"/></body></html></p>
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<p><html><body><img src="https://static.igem.org/mediawiki/parts/8/84/DT_dppiv_model.PNG"/></body></html></p>
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<p><html><body><img src="https://static.igem.org/mediawiki/parts/f/fd/DC_dppiv_model.PNG"/></body></html></p>
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<p><html><body><img src="https://static.igem.org/mediawiki/parts/9/95/DG_dppiv_model.PNG"/></body></html></p>
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<p>Where R<sub>i</sub>=concentration of interfering RNA</p>
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<p>T=concentration of target RNA</p>
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<p>R<sub>ds</sub>=concentration of double stranded RNA (made from interfering RNA and target association)</p>
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<p>C=siRNA/RISC complex</p>
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<p>and G=GFP expression</p>

Latest revision as of 23:24, 15 October 2014

DPP-IV (our siRNA target)


DPP-IV 3'UTR (target of the siRNA)

As proof of concept for our project we envisaged downregulating DPP-IV production by interfering with the translational part of its production, through RNA interference.This is a section of the 3’UTR of the protein dipeptidyl peptidase four (DPP-IV). This contains the target sequence for the siRNA (BBa_K1442113).


DPPIV.jpg The schematic above shows the organisation of the DPP-IV target

Usage

This was used to test the silencing ability of the siRNA for DPP-IV (BBa_K1142113) by cloning the target into the forwards GFP for humans (BBa_K1442106). A decrease in fluorescence would be observed with silencing.

Model

Where Ri=concentration of interfering RNA

T=concentration of target RNA

Rds=concentration of double stranded RNA (made from interfering RNA and target association)

C=siRNA/RISC complex

and G=GFP expression