Difference between revisions of "Part:BBa I11012:Experience"

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iGEM14_Edinburgh added this tag to RFP ([[Part:BBa_K1399000]]) and constructed lactose/IPTG inducible measurement pathway  ([[Part:BBa_K1399010]]) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)
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[[File:Tagged RFP min and max values at different temperatures.jpg|600px|thumb|center|'''SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).''']] [[File:EDiGEM14 RFP legend.png|500px|thumb|center|'''Summary of BioBricks to which our characterization data is applicable.'''
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<I>Edinburgh IGEM 2014</I>
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iGEM14_Edinburgh added this tag to RFP ([[Part:BBa_K1399000]]) and constructed lactose/IPTG inducible measurement pathway  ([[Part:BBa_K1399010]]) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)
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[[File:Tagged RFP min and max values at different temperatures.jpg|600px|thumb|center|'''SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).''']]
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[[File:EDiGEM14 RFP legend.png|500px|thumb|center|'''Summary of BioBricks to which our characterization data is applicable.''']]
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Revision as of 23:15, 15 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I11012

User Reviews

UNIQ219dddfe2e9239db-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

UNIQ219dddfe2e9239db-partinfo-00000002-QINU

No review score entered. Edinburgh IGEM 2014

iGEM14_Edinburgh added this tag to RFP (Part:BBa_K1399000) and constructed lactose/IPTG inducible measurement pathway (Part:BBa_K1399010) to characterize and compare the instability of this SsrA-AAV tagged RFP to others containing different variants of SsrA tags. We determined the steady state fluorescence/OD ratio of E. coli JM109 at uninduced (minimum steady state) and induced (maximum steady state) state. To see how the temperature of environment can affect protein degradation efficiency we repeated experiments in several temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C). Our data are summarised below. (For more information on SsrA degradation tag system, our experimental assays and results please visit our wiki page [http://2014.igem.org/Team:Edinburgh].)

SsrA degron tagged RFP minimum (left) and maximum (right) fluorescence per OD at four different temperatures (28⁰C, 32⁰C, 37⁰C and 42⁰C).
Summary of BioBricks to which our characterization data is applicable.
;