Difference between revisions of "Part:BBa K1442106"
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== FG == | == FG == | ||
| − | The forward GFP for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells | + | <p>The forward GFP testing module for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells using IDT codon optimiser and was used to test the EMCV IRES, NKRF IRES and Anti-theophylline aptazyme in vivo.<p> |
[[File:FG.jpg]] | [[File:FG.jpg]] | ||
''This schematic developed in Geneious shows the organisation of the FG testing module'' | ''This schematic developed in Geneious shows the organisation of the FG testing module'' | ||
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== Usage == | == Usage == | ||
The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111). | The FG was intended to be used to test the EMCV IRES (BBa_K1442023) and the NKRF IRES (BBa_K1442024) using the restriction sites AscI and BsrGI before the GFP. After the GFP are two more restriction sites Mau BI and Mlu I that can be used to test the Aptazyme (BBa_K1442006) and the DPP-IV target (BBa_K1442111). | ||
Latest revision as of 22:57, 15 October 2014
Forward GFP for human cells
FG
The forward GFP testing module for human cells is a specially designed testing module. It contains GFP codon optimised to work in human cells using IDT codon optimiser and was used to test the EMCV IRES, NKRF IRES and Anti-theophylline aptazyme in vivo.<p>
This schematic developed in Geneious shows the organisation of the FG testing module