Difference between revisions of "Part:BBa K1442308:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 10: Line 10:
 
<p>This contains restriction sites in order to insert  testing modules either side of the forwards GFP.</p>
 
<p>This contains restriction sites in order to insert  testing modules either side of the forwards GFP.</p>
 
<p>The stop codons were artificially added in order to definitively stop translation following this.</p>
 
<p>The stop codons were artificially added in order to definitively stop translation following this.</p>
 +
<p>THe poly A tail was added to ensure efficient transfection of the mRNA following in vitro transcription.</p>
  
 
===Source===
 
===Source===

Latest revision as of 22:49, 15 October 2014


GFP codon optimised for human cells


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 44


Design Notes

Codon optimised using IDT codon optimiser

This contains restriction sites in order to insert testing modules either side of the forwards GFP.

The stop codons were artificially added in order to definitively stop translation following this.

THe poly A tail was added to ensure efficient transfection of the mRNA following in vitro transcription.

Source

Part BBa_E0040

References