Difference between revisions of "Part:BBa K1399015"
 
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Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
 
Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator.
−
The tagged GFP is actively degraded within cell, thus provides better temporal resolution of green fluorescence and promoter activity.
+
The tagged GFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of green fluorescence and promoter activity.
  
 
This part can be considered as improved version of [[Part:BBa_J04431]] as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag.
 
This part can be considered as improved version of [[Part:BBa_J04431]] as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag.
  
 +
[[File:EDiGEM14-SsrA degron mediated degradation.png|400px|thumb|center|'''Figure 1 SsrA degron mediated protein degradation.''' ('''A''') Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). ('''B''') The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 22:43, 15 October 2014

Plac-GFP(LVA)

Lactose/IPTG inducible promoter with GFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged GFP is actively degraded within cell (Figure 1), thus provides better temporal resolution of green fluorescence and promoter activity.

This part can be considered as improved version of Part:BBa_J04431 as it does not contain StuI restriction site coding for additional 'RP' residues between GFP and LVA tag.

Figure 1 SsrA degron mediated protein degradation. (A) Any version of SsrA tags can be attached to any protein of interest (e.g. RFP or GFP). (B) The tag is recognized by ClpX unfoldase forming a complex with ClpP protease and the tagged protein gets degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870