Difference between revisions of "Part:BBa K1475002:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1475002 short</partinfo>
 
<partinfo>BBa_K1475002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We used primers to add prefix and suffix to the coding part.
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Synthesized from the coding sequence of isoform a, sequence avaliable on uniprot.org. [3]
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Prefix and suffix were added using the following primers.
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<br>
 +
Forward:
 +
<br>
 +
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC
 +
<br>
 +
Rewerse:
 +
<br>
 +
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC
  
  
  
 
===Source===
 
===Source===
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Organizm: ''Caenorhabditis elegans''
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The cDNA sequence comes from wormbase.org and was generously provided by Julius Fredens from University of Southern Denmark.
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The gene was synthesized based on the cDNA sequence from wormbase.org and was generously provided by Julius Fredens from University of Southern Denmark. [3]
  
 
===References===
 
===References===
 +
1. Xue-Rong Zhou, et al.: Caenorhabditis elegans Δ12-Desaturase FAT-2 Is a Bifunctional Desaturase Able to Desaturate a Diverse Range of Fatty Acid Substrates at the Δ12 and Δ15 Positions. J Biol Chem. Dec 23, 2011; 286(51): p. 43644–43650, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243535/
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<br>
 +
2. Wormbase, 10 Jun 2010: fat-2. http://www.wormbase.org/species/c_elegans/gene/WBGene00001394?query=fat-2#0-9e-3.
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<br>
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3. Uniprot, fat-2_CAEEL accession number: G5EGA5. http://www.uniprot.org/uniprot/G5EGA5

Revision as of 21:31, 15 October 2014

FAT-2 delta 12 desaturase from C. elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
    Illegal BamHI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 169
    Illegal SapI site found at 582


Design Notes

Synthesized from the coding sequence of isoform a, sequence avaliable on uniprot.org. [3] Prefix and suffix were added using the following primers.
Forward:
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC
Rewerse:
AAGAATTCGCGGCCGCTTCTAGATGACAATCGCTACAAAAGTGAAC


Source

Organizm: Caenorhabditis elegans The gene was synthesized based on the cDNA sequence from wormbase.org and was generously provided by Julius Fredens from University of Southern Denmark. [3]

References

1. Xue-Rong Zhou, et al.: Caenorhabditis elegans Δ12-Desaturase FAT-2 Is a Bifunctional Desaturase Able to Desaturate a Diverse Range of Fatty Acid Substrates at the Δ12 and Δ15 Positions. J Biol Chem. Dec 23, 2011; 286(51): p. 43644–43650, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243535/
2. Wormbase, 10 Jun 2010: fat-2. http://www.wormbase.org/species/c_elegans/gene/WBGene00001394?query=fat-2#0-9e-3.
3. Uniprot, fat-2_CAEEL accession number: G5EGA5. http://www.uniprot.org/uniprot/G5EGA5