Difference between revisions of "Part:BBa K1475003:Design"
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===Design Notes===
 
===Design Notes===
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The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_K1475003 Part:BBa_C0040]) using the following primers:
+
The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_K1475003 Part:BBa_C0040]) using the following primers.
 +
<br>
 
Forward primer:  
 
Forward primer:  
 
<br>
 
<br>
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===Source===
 
===Source===
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Organizm: ''Escherichia coli''
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−
This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.
 
  
 
===References===
 
===References===

Revision as of 20:31, 15 October 2014

tetracyclin repressor from transposon Tn10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers.
Forward primer:
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer:
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC



Source

Organizm: Escherichia coli


References