Difference between revisions of "Part:BBa K1475003:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1475003 short</partinfo>
 
<partinfo>BBa_K1475003 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Skrive hvilke primere der er brugt.
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The part was constructed by PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_K1475003 Part:BBa_C0040]) using the following primers:
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Forward primer:
 +
<br>
 +
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
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<br>
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Reverse primer:
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<br>
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TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC
  
  

Revision as of 20:30, 15 October 2014

tetracyclin repressor from transposon Tn10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was constructed by PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040) using the following primers: Forward primer:
AAGAATTCGCGGCCGCTTCTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAACAGCG
Reverse primer:
TTTCTGCAGCGGCCGCTACTAGTATTATTAGGACCCACTTTCACATTTAAGTTGTTTTTC



Source

This part was constructed from the tetracycline repressor from transposon Tn10 (+LVA) (Part:BBa_C0040) using primers to remove the LVA protein digestion tag. That is THIS PART DOES NOT CONTAIN THE LVA PROTEIN DIGESTION TAG.

References