Difference between revisions of "Part:BBa K1475010"

 
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<partinfo>BBa_K1475010 short</partinfo>
 
<partinfo>BBa_K1475010 short</partinfo>
  
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This part is the limonene generator with tet promoter. The limonene generator consists of deoxyxylulose phosphtae synthase (dxs), limonene synthase (LIMS1) and appY.
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This part contains the dxs gene from Escherichia coli encoding the thiamine-diphosphate dependant 1-deoxyxylulose-5-phosphate synthase, the LIMS1 gene from Citrus limon encoding (R)-limonene synthase ([http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]) and the appY ([http://www.uniprot.org/uniprot/P05052 UniProt:P05052]) gene from Escherichia coli encoding a transcriptional regulator related to anaerobic metabolism. These genes are under the control of the TetR promoter.
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<br>
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Overexpression of 1-deoxyxylulose-5-phosphate synthase and appY has been shown to increase production of carotenoids and other terpenoids [1] (limonene is a terpenoid) and has thus been included in this part by the 2008 Edinburgh team, find the original part here: [https://parts.igem.org/Part:BBa_K118024 Part:BBa_K118024].
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===Usage and Biology===
 
===Usage and Biology===
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The dxs gene from Escherichia coli encodes the 1-deoxyxylulose-5-phosphate synthase, which requires thiamine-diphosphate to function. It catalyses the conversion of pyruvate and D-glyceraldehyde 3-phosphate into 1-deoxy-D-xylulose 5-phosphate and CO2. [1][2]
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The LIMS1 gene encodes the (R)-limonene synthase from Citrus limon. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [3]
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appY is a transcriptional regulator that induces the transcription of acid phosphatase (appA) together with other proteins. appY transcriptional repression functions. [4]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1475010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1475010 SequenceAndFeatures</partinfo>

Revision as of 20:05, 15 October 2014

dxs+LIMS1+appY regulated by the tet promoter

This part contains the dxs gene from Escherichia coli encoding the thiamine-diphosphate dependant 1-deoxyxylulose-5-phosphate synthase, the LIMS1 gene from Citrus limon encoding (R)-limonene synthase ([http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]) and the appY ([http://www.uniprot.org/uniprot/P05052 UniProt:P05052]) gene from Escherichia coli encoding a transcriptional regulator related to anaerobic metabolism. These genes are under the control of the TetR promoter.
Overexpression of 1-deoxyxylulose-5-phosphate synthase and appY has been shown to increase production of carotenoids and other terpenoids [1] (limonene is a terpenoid) and has thus been included in this part by the 2008 Edinburgh team, find the original part here: Part:BBa_K118024.


Usage and Biology

The dxs gene from Escherichia coli encodes the 1-deoxyxylulose-5-phosphate synthase, which requires thiamine-diphosphate to function. It catalyses the conversion of pyruvate and D-glyceraldehyde 3-phosphate into 1-deoxy-D-xylulose 5-phosphate and CO2. [1][2]

The LIMS1 gene encodes the (R)-limonene synthase from Citrus limon. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [3]

appY is a transcriptional regulator that induces the transcription of acid phosphatase (appA) together with other proteins. appY transcriptional repression functions. [4]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808
    Illegal SapI.rc site found at 2068