Difference between revisions of "Part:BBa K1456003"

 
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<partinfo>BBa_K1456003 short</partinfo>
 
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This enzyme degrades oxygen radicals.
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  <li><b>SOD is a powerful and essential antioxidant enzyme</b> which converts and scavenges free radicals and reactive oxygen species (ROS) into hydrogen peroxide (H2O2), slightly less harmful compound in order to convey it to the second step reaction which detoxifies H2O2 into water. (Batelli et al. 1972; Baudry et al. 1993; Gonzalez et al. 1995; Doctrow et al. 1996) </li>
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<li>In our body cells, there are three different subtypes of SOD enzyme in different cellular levels. SOD-1 exists in cytoplasm, SOD-2 in mitochondria and SOD-3 is in extracellular matrix. We have chosen SOD-1 to take advantage of easy interference and activity capacity due to its location. Additionally, <b>there is evidence in the literature for the synthetic production of SOD-1 enzyme by transfecting human cells.</b> (Shuvaev et al. 2013)</li>
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"https://static.igem.org/mediawiki/2014/thumb/4/40/ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg/800px-ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg"
  
 
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Revision as of 19:32, 15 October 2014

Superoxide dismutase-1 (SOD-1)

  • SOD is a powerful and essential antioxidant enzyme which converts and scavenges free radicals and reactive oxygen species (ROS) into hydrogen peroxide (H2O2), slightly less harmful compound in order to convey it to the second step reaction which detoxifies H2O2 into water. (Batelli et al. 1972; Baudry et al. 1993; Gonzalez et al. 1995; Doctrow et al. 1996)
  • In our body cells, there are three different subtypes of SOD enzyme in different cellular levels. SOD-1 exists in cytoplasm, SOD-2 in mitochondria and SOD-3 is in extracellular matrix. We have chosen SOD-1 to take advantage of easy interference and activity capacity due to its location. Additionally, there is evidence in the literature for the synthetic production of SOD-1 enzyme by transfecting human cells. (Shuvaev et al. 2013)

"800px-ATOMS-Biobricks-XO-SOD-GPx-Aprotinin.jpg"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 327