Difference between revisions of "Part:BBa K1554004:Design"

(→‎Design Notes)
(→‎Source)
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Barnase [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173]
 
Barnase [https://parts.igem.org/Part:BBa_J72173 Part:BBa_J72173]
  
−
AmilGFP [Waiting for Norwich team part name in registry]
+
AmilGFP [https://parts.igem.org/Part:BBa_J72173 Part:BBa_K1467201]
  
 
P35s [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
 
P35s [https://parts.igem.org/Part:BBa_K1537015 Part:BBa_K1537015]
 +
 +
T35s: GoldenBraid 2.0 Collection
  
 
===References===
 
===References===

Revision as of 18:56, 15 October 2014


Yellow biosafety module for plants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.

Source

TA29 promoter Part:BBa_K1554000

Barnase Part:BBa_J72173

AmilGFP Part:BBa_K1467201

P35s Part:BBa_K1537015

T35s: GoldenBraid 2.0 Collection

References