Difference between revisions of "Part:BBa K1342001"
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===TLC Assay=== | ===TLC Assay=== | ||
[[File:TLC_20140929_.jpg|200px|thumb|Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)|none]] | [[File:TLC_20140929_.jpg|200px|thumb|Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)|none]] | ||
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| − | [[File:TLC_intensity_of_the_band.jpg]] | + | |
| + | [[File:TLC_intensity_of_the_band.jpg|300px]] | ||
Revision as of 18:43, 15 October 2014
zinTp_AspA
ZinTp_AspA contains ZinTp(BBa_K1342005), RBS(BBa_B0034), ApeA(BBa_K1342002), Dter(BBa_B0015). AspA is expressed in the presence of cadmium ion. And translated to aspartase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 179
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1071
Illegal AgeI site found at 600
Illegal AgeI site found at 1452 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1111
TLC Assay
Analysis of aspartate from strain E.coli K-12/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, not addition cadmium to previous culture of synthesis medium (diluted 1:50 by synthesis medium) 2, (diluted 1:100 by synthesis medium) 3, add cadmium (250μM) 4, (diluted 1:50 by synthesis medium) 5, (diluted 1:100 by synthesis medium) 6, (diluted 1:200 by synthesis medium)
