Difference between revisions of "Part:BBa K1554000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part was obtaining by PCR amplification using Phusion polymerase and afterwards sequenced. | |
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===Source=== | ===Source=== | ||
Revision as of 18:34, 15 October 2014
TA29 promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was obtaining by PCR amplification using Phusion polymerase and afterwards sequenced.
Source
Amplified by PCR from genomic DNA of Nicotiana tabacco.
References
[1] Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., and Goldberg, R.B. (1990). lnduction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 347, 737–741.
[2] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333
[3] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278
[4] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130
[5] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.